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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P2RX4 Monoclonal antibody (66416-1-Ig)

    WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using P2RX4 Monoclonal antibody (66416-1-Ig)

    WB analysis using 66416-1-Ig (same clone as 66416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using P2RX4 Monoclonal antibody (66416-1-Ig)

    WB analysis of mouse brain using 66416-1-Ig (same clone as 66416-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using P2RX4 Monoclonal antibody (66416-1-Ig)

    WB analysis of rat brain using 66416-1-Ig (same clone as 66416-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using P2RX4 Monoclonal antibody (66416-1-Ig)

    IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using P2RX4 Monoclonal antibody (66416-1-Ig)

    IHC staining of human kidney using 66416-1-Ig (same clone as 66416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66416-1-PBS in a different storage buffer formulation.

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P2RX4 Monoclonal antibody, PBS Only

P2RX4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66416-1-PBS
Clone No.1A5A6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66416-1-PBS targets P2RX4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P2RX4 fusion protein Ag4445 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2X, ligand-gated ion channel, 4
Calculated molecular weight 388 aa, 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC033826
Gene Symbol P2RX4
Gene ID (NCBI) 5025
RRIDAB_2881788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).