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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RX5 Polyclonal antibody, PBS Only

P2RX5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19012-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using P2RX5 Polyclonal antibody (19012-1-AP)

    WB analysis of BxPC-3 using 19012-1-AP (same clone as 19012-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using P2RX5 Polyclonal antibody (19012-1-AP)

    WB analysis of HL-60 using 19012-1-AP (same clone as 19012-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using P2RX5 Polyclonal antibody (19012-1-AP)

    WB analysis of MDA-MB-453s using 19012-1-AP (same clone as 19012-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using P2RX5 Polyclonal antibody (19012-1-AP)

    IF Staining of BxPC-3 using 19012-1-AP (same clone as 19012-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 19012-1-AP (P2RX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19012-1-PBS targets P2RX5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX5 fusion protein Ag5297 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Calculated molecular weight 422 aa, 47 kDa
Observed molecular weight 44 kDa, 49 kDa, 116 kDa
GenBank accession numberBC039015
Gene symbol P2RX5
Gene ID (NCBI) 5026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.