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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RX5 Polyclonal antibody, PBS Only

P2RX5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19012-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using P2RX5 Polyclonal antibody (19012-1-AP)

    WB analysis of BxPC-3 using 19012-1-AP (same clone as 19012-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using P2RX5 Polyclonal antibody (19012-1-AP)

    WB analysis of HL-60 using 19012-1-AP (same clone as 19012-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using P2RX5 Polyclonal antibody (19012-1-AP)

    WB analysis of MDA-MB-453s using 19012-1-AP (same clone as 19012-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using P2RX5 Polyclonal antibody (19012-1-AP)

    IF Staining of BxPC-3 using 19012-1-AP (same clone as 19012-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 19012-1-AP (P2RX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19012-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19012-1-PBS targets P2RX5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX5 fusion protein Ag5297 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Calculated molecular weight 422 aa, 47 kDa
Observed molecular weight 44 kDa, 49 kDa, 116 kDa
GenBank accession numberBC039015
Gene symbol P2RX5
Gene ID (NCBI) 5026
RRIDAB_2299079
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.