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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RX6 Recombinant antibody

P2RX6 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241620B10

Cat no : 84458-4-RR

Synonyms

P2XM, P2X6, P2X purinoceptor 6, P2RXL1, ATP receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using P2RX6 Recombinant antibody (84458-4-RR)

    WB analysis of mouse skeletal muscle using 84458-4-RR

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84458-4-RR (P2RX6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84458-4-RR

    Affinity and Kinetic Characterization of 84458-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84458-4-RR against Human P2RX6 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84458-4-RR is P2RX6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P2RX6 fusion protein Ag12331 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2X, ligand-gated ion channel, 6
Calculated molecular weight 431 aa, 48 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC033488
Gene symbol P2RX6
Gene ID (NCBI) 9127
RRIDAB_3671983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The P2 receptors have two subfamilies in mammalian cells, P2X and P2Y. P2X family of ligand-gated ion channel receptors have seven members, P2RX1-7, which all constitute non-selective cation channels proposed to be functional under a homo- or heterotrimeric association. P2RX6 is predominantly expressed in skeletal muscle, and regulated by p53. It's reported that ATP might increase renal cell carcinoma cells migration and invasion through P2RX6.(PMID: 31159832, PMID:9242461)

Protocols

Product Specific Protocols
WB protocol for P2RX6 antibody 84458-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols