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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RY1 Monoclonal antibody, PBS Only

P2RY1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1F3

Cat no : 67654-1-PBS

Synonyms

ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P2RY1 Monoclonal antibody (67654-1-Ig)

    WB analysis using 67654-1-Ig (same clone as 67654-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67654-1-Ig (P2RY1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IHC staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IHC staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IHC staining of human placenta using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IHC staining of human placenta using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IF Staining of rat brain using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IF Staining of rat brain using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IF Staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using P2RY1 Monoclonal antibody (67654-1-Ig)

    IF Staining of mouse brain using 67654-1-Ig (same clone as 67654-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67654-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67654-1-PBS targets P2RY1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P2RY1 fusion protein Ag12762 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2Y, G-protein coupled, 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC074785
Gene symbol P2RY1
Gene ID (NCBI) 5028
RRIDAB_2882853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.