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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RY13 Polyclonal antibody

P2RY13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20335-1-AP

Synonyms

G-protein coupled receptor 94, G-protein coupled receptor 86, GPR94, GPR86, GPCR1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using P2RY13 Polyclonal antibody (20335-1-AP)

    WB analysis of HeLa using 20335-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20335-1-AP (P2RY13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using P2RY13 Polyclonal antibody (20335-1-AP)

    WB analysis of HepG2 using 20335-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20335-1-AP (P2RY13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using P2RY13 Polyclonal antibody (20335-1-AP)

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using P2RY13 Polyclonal antibody (20335-1-AP)

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20335-1-AP targets P2RY13 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RY13 fusion protein Ag14108 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2Y, G-protein coupled, 13
Calculated molecular weight 354 aa, 41 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC041116
Gene symbol P2RY13
Gene ID (NCBI) 53829
RRIDAB_2878674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P2RY13 (also named GPR86 or GPR94) is a G protein-coupled receptor (GPCR), which is involved in the pathogenesis of inflammation and immune disorders (PMID: 35982893). Several studies have reported that P2RY13 is a key regulator of cholesterol transport and hepatic HDL endocytosis and is involved in bone formation, remodeling, cell survival, and neuroprotection (PMID: 38045624).

Protocols

Product Specific Protocols
WB protocol for P2RY13 antibody 20335-1-APDownload protocol
IHC protocol for P2RY13 antibody 20335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Health Sci Rep

P2RY13 is a prognostic biomarker and associated with immune infiltrates in renal clear cell carcinoma: A comprehensive bioinformatic study

Authors - Jie Chu
View Article
humanWB

Nat Methods

SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes

Authors - Ruiqiao He
View Article