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  • KD/KO Validated

P4HA2 Polyclonal antibody

P4HA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 13759-1-AP

Synonyms

4 PH alpha 2, P4HA2


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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13759-1-AP targets P4HA2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P4HA2 fusion protein Ag4714 相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, alpha polypeptide II
Calculated molecular weight 533 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC035813
Gene symbol P4HA2
Gene ID (NCBI) 8974
RRIDAB_2156286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for P4HA2 antibody 13759-1-APDownload protocol
IHC protocol for P4HA2 antibody 13759-1-APDownload protocol
IP protocol for P4HA2 antibody 13759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Hepatology

P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases

Authors - Jun Zhang
  • KD Validated
humanIHC

Blood

Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin.

Authors - Wei Jiang

Nat Commun

Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis.

Authors - Kerrie L Marie
humanWB

EBioMedicine

Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.

Authors - Tianjiao Wang
humanWB

Cancer Res

Epigenetic SMAD3 repression in tumor-associated fibroblasts impairs fibrosis and response to the antifibrotic drug nintedanib in lung squamous cell carcinoma.

Authors - Rafael Ikemori
humanWB,IHC

Oncogene

Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.

Authors - G X Feng