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P4HA2 Polyclonal antibody
P4HA2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 13759-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human cervical cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 6 publications below |
WB | See 16 publications below |
IHC | See 8 publications below |
IF | See 4 publications below |
Product Information
13759-1-AP targets P4HA2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | P4HA2 fusion protein Ag4714 相同性解析による交差性が予測される生物種 |
Full Name | prolyl 4-hydroxylase, alpha polypeptide II |
Calculated molecular weight | 533 aa, 61 kDa |
Observed molecular weight | 61 kDa |
GenBank accession number | BC035813 |
Gene symbol | P4HA2 |
Gene ID (NCBI) | 8974 |
RRID | AB_2156286 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for P4HA2 antibody 13759-1-AP | Download protocol |
IHC protocol for P4HA2 antibody 13759-1-AP | Download protocol |
IP protocol for P4HA2 antibody 13759-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hepatology P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases
| ||
Blood Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin. | ||
Nat Commun Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis. | ||
EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. | ||
Cancer Res Epigenetic SMAD3 repression in tumor-associated fibroblasts impairs fibrosis and response to the antifibrotic drug nintedanib in lung squamous cell carcinoma. | ||
Oncogene Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA. |