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P4HA2 Monoclonal antibody
P4HA2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3A9A4
Cat no : 66604-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HSCT6 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66604-1-Ig targets P4HA2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
| Tested Reactivity | Human, Mouse , Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P4HA2 fusion protein Ag4714 相同性解析による交差性が予測される生物種 |
| Full Name | prolyl 4-hydroxylase, alpha polypeptide II |
| Calculated molecular weight | 533 aa, 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC035813 |
| Gene symbol | P4HA2 |
| Gene ID (NCBI) | 8974 |
| RRID | AB_2881964 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P4HA2 antibody 66604-1-Ig | Download protocol |
| IHC protocol for P4HA2 antibody 66604-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion. | ||
Cell Commun Signal GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement | ||
Cancer Manag Res Prolyl-4-Hydroxylase α Subunit 2 as a Novel Potential Biomarker for Predicting the Prognosis of Epithelial Ovarian Carcinoma. | ||
NAR Mol Med C G composition in transposon-derived genes is increased in FXD with perturbed immune system |
