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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis using 60283-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of HSC-T6 using 60283-2-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of ROS1728 using 60283-2-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat thymus tissue using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of rat thymus using 60283-2-Ig

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of COLO 320 using 60283-2-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of HSC-T6 using 60283-2-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of HSC-T6 using 60283-2-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of COLO 320 using 60283-2-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of COLO 320 using 60283-2-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of HSC-T6 using 60283-2-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using P53 Monoclonal antibody (60283-2-Ig)

    WB analysis of ROS1728 using 60283-2-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human ovary cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human tonsillitis using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human tonsillitis using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human ovary tumor using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human ovary tumor using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human gliomas using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human gliomas using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human colon cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human lung cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human lung cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human breast cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human breast cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human stomach cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using P53 Monoclonal antibody (60283-2-Ig)

    IHC staining of human stomach cancer using 60283-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using P53 Monoclonal antibody (60283-2-Ig)

    IF Staining of A431 using 60283-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

P53 Monoclonal antibody

P53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60283-2-Ig
Clone No.6C4B6
Publications(270)

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, COLO 320 cells, HSC-T6 cells, ROS1728 cells, rat thymus tissue, HaCaT cells, HT-29 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human gliomas tissue, human lung cancer tissue, human ovary cancer tissue, human ovary tumor tissue, human stomach cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1600-1:6400
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60283-2-Ig targets P53 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, rat, pig, rabbit, canine, zebrafish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P53 fusion protein Ag0698 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
RRIDAB_2881401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.


Protocols

Product Specific Protocols
WB protocol for P53 antibody 60283-2-IgDownload protocol
IHC protocol for P53 antibody 60283-2-IgDownload protocol
IF protocol for P53 antibody 60283-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Bioact Mater

Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma

Authors - Yuhan Wang
View Article
mouseWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
View Article
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
View Article
mouseIHC

Aging (Albany NY)

Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging

Authors - Xue Wang
View Article
WB

J Adv Res

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway

Authors - Kai Sun
View Article
humanWB

Clin Transl Med

p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer

Authors - Junjing Zhang
View Article