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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P53 Recombinant antibody (80077-1-RR)

    WB analysis using 80077-1-RR (same clone as 80077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using P53 Recombinant antibody (80077-1-RR)

    WB analysis of zebrafish using 80077-1-RR (same clone as 80077-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using P53 Recombinant antibody (80077-1-RR)

    WB analysis of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using P53 Recombinant antibody (80077-1-RR)

    WB analysis of ROS1728 using 80077-1-RR (same clone as 80077-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using P53 Recombinant antibody (80077-1-RR)

    WB analysis of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW480 cells using P53 Recombinant antibody (80077-1-RR)

    WB analysis of SW480 using 80077-1-RR (same clone as 80077-1-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using P53 Recombinant antibody (80077-1-RR)

    WB analysis of HT-29 using 80077-1-RR (same clone as 80077-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P53 Recombinant antibody (80077-1-RR)

    IHC staining of human colon cancer using 80077-1-RR (same clone as 80077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using P53 Recombinant antibody (80077-1-RR)

    IF Staining of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using P53 Recombinant antibody (80077-1-RR)

    IF Staining of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using P53 Recombinant antibody (80077-1-RR)

    FC experiment of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

P53 Recombinant antibody, PBS Only

P53 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80077-1-PBS
Clone No.2H13

Host / Isotype

Rabbit / IgG

Reactivity

human, zebrafish

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

TP53, 2H13, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80077-1-PBS targets P53 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, zebrafish samples.

Tested Reactivity human, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight53 kDa
GenBank accession numberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
RRIDAB_2918862
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04637
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)