Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, mouse colon tissue, rat colon tissue, A549 cells, MCF-7 cells, HeLa cells, LNCaP cells, HepG2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
68184-1-Ig targets P5CS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P5CS fusion protein Ag12100 相同性解析による交差性が予測される生物種 |
| Full Name | aldehyde dehydrogenase 18 family, member A1 |
| Calculated molecular weight | 795 aa, 87 kDa |
| Observed molecular weight | 87 kDa |
| GenBank accession number | BC106054 |
| Gene Symbol | P5CS |
| Gene ID (NCBI) | 5832 |
| RRID | AB_2935275 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54886 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P5CS antibody 68184-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|