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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P5CS Monoclonal antibody

P5CS Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B1E11

Cat no : 68184-1-Ig

Synonyms

ALDH18A1, Gamma-glutamyl phosphate reductase, Gamma-glutamyl kinase, Delta-1-pyrroline-5-carboxylate synthase, Aldehyde dehydrogenase family 18 member A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P5CS Monoclonal antibody (68184-1-Ig)

    WB analysis using 68184-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of mouse colon tissue using P5CS Monoclonal antibody (68184-1-Ig)

    WB analysis of mouse colon using 68184-1-Ig

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using P5CS Monoclonal antibody (68184-1-Ig)

    WB analysis of rat colon using 68184-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU2OS cells, mouse colon tissue, rat colon tissue, A549 cells, MCF-7 cells, HeLa cells, LNCaP cells, HepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68184-1-Ig targets P5CS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P5CS fusion protein Ag12100 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 18 family, member A1
Calculated molecular weight 795 aa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC106054
Gene symbol P5CS
Gene ID (NCBI) 5832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for P5CS antibody 68184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols