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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31403-1-AP

Synonyms

P62/SQSTM1, p62, Sqstm1, sequestosome 1, Osi

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Polyclonal antibody (31403-1-AP)

    WB analysis using 31403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Polyclonal antibody (31403-1-AP)

    WB analysis using 31403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using P62,SQSTM1 Polyclonal antibody (31403-1-AP)

    IF Staining of HepG2 using 31403-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using P62,SQSTM1 antibody (31403-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, RAW 264.7 cells
Positive IF/ICC detected inChloroquine treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31403-1-AP targets P62,SQSTM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag35064 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight48 kDa
Observed molecular weight 62 kDa
GenBank accession numberNM_011018
Gene symbol P62/SQSTM1
Gene ID (NCBI) 18412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

Protocols

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 31403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols