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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    WB analysis of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    WB analysis of HepG2 using 80294-1-RR (same clone as 80294-1-PBS)

    WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HepG2 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    IP experiment of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    IP result of anti-P62,SQSTM1 (IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    IHC staining of human pancreas cancer using 80294-1-RR (same clone as 80294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    IHC staining of human stomach cancer using 80294-1-RR (same clone as 80294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    IF Staining of HeLa using 80294-1-RR (same clone as 80294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using P62,SQSTM1 Recombinant antibody (80294-1-RR)

    FC experiment of Jurkat using 80294-1-RR (same clone as 80294-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (80294-1-RR, Clone:1D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

P62,SQSTM1 Recombinant antibody, PBS Only

P62,SQSTM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80294-1-PBS
Clone No.1D17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

P62/SQSTM1, P62, SQSTM1, Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80294-1-PBS targets P62,SQSTM1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight62 kDa
GenBank accession numberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2918883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13501
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).