P62,SQSTM1 Recombinant antibody, PBS Only

Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HepG2 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

IP result of anti-P62,SQSTM1 (IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (80294-1-RR, Clone:1D17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.