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  • Featured Product
  • KD/KO Validated

P62,SQSTM1 Recombinant antibody, PBS Only

P62,SQSTM1 Recombinant Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D17

Cat no : 80294-1-PBS

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

Validation Data Gallery

Applications:
  • WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis of HepG2 using 80294-1-RR (same clone as 80294-1-PBS)

    WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HepG2 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    IP experiment of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    IP result of anti-P62,SQSTM1 (IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 80294-1-RR (same clone as 80294-1-PBS)

    IHC staining of human pancreas cancer using 80294-1-RR (same clone as 80294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 80294-1-RR (same clone as 80294-1-PBS)

    IHC staining of human stomach cancer using 80294-1-RR (same clone as 80294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80294-1-RR (same clone as 80294-1-PBS)

    IF Staining of HeLa using 80294-1-RR (same clone as 80294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80294-1-RR (same clone as 80294-1-PBS)

    FC experiment of Jurkat using 80294-1-RR (same clone as 80294-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (80294-1-RR, Clone:1D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

80294-1-PBS targets P62,SQSTM1 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).