Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-80294 targets P62,SQSTM1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種 |
| Full Name | sequestosome 1 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 62 kDa |
| GenBank accession number | BC017222 |
| Gene Symbol | P62/SQSTM1 |
| Gene ID (NCBI) | 8878 |
| RRID | AB_3084488 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13501 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 P62,SQSTM1 antibody CL488-80294 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |