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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P85a; PIK3R1 Polyclonal antibody

P85a; PIK3R1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30092-1-AP

Synonyms

GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P85a; PIK3R1 Polyclonal antibody (30092-1-AP)

    WB analysis using 30092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using P85a; PIK3R1 Polyclonal antibody (30092-1-AP)

    WB analysis using 30092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30092-1-AP targets P85a; PIK3R1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC030815
Gene symbol PI3 Kinase p85 Alpha
Gene ID (NCBI) 5295
RRIDAB_3086227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for P85a; PIK3R1 antibody 30092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols