Featured Product
KD/KO Validated

PA2G4 Polyclonal antibody

Name: PA2G4 Polyclonal antibody
Brand: Proteintech
SKU: 15348-1-AP

PA2G4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15348-1-AP

Synonyms

hG4-1, HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 15348-1-AP
WB result of PA2G4 antibody (15348-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.
WB analysis using 15348-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 15348-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 15348-1-AP
IP result of anti-PA2G4 (IP:15348-1-AP, 4ug; Detection:15348-1-AP 1:1000) with Jurkat cells lysate 3000ug.
IHC staining of human breast cancer using 15348-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15348-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15348-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PA2G4 antibody (15348-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

-/ -

Cat No. 15348-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, HEK-293 cells, rat spleen tissue, HL-60 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

15348-1-AP targets PA2G4 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PA2G4 fusion protein Ag7587 相同性解析による交差性が予測される生物種
Full Name	proliferation-associated 2G4, 38kDa
Calculated molecular weight	44 kDa
Observed molecular weight	42 kDa, 48 kDa
GenBank accession number	BC001951
Gene symbol	PA2G4
Gene ID (NCBI)	5036
RRID	AB_2156602
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.

Protocols

Product Specific Protocols
WB protocol for PA2G4 antibody 15348-1-AP	Download protocol
IHC protocol for PA2G4 antibody 15348-1-AP	Download protocol
IF protocol for PA2G4 antibody 15348-1-AP	Download protocol
IP protocol for PA2G4 antibody 15348-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF,RIP	Cell Biosci PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner. Authors - Sheng Sun KD Validated View Article
human	WB,IHC	FASEB J Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency Authors - Roseanne Rosario View Article
		Methods Enzymol Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells Authors - Manuel Günnigmann View Article
human	WB,IF,IP	J Dent Sci Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma Authors - Legang Sun View Article
human	WB	Nat Commun Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML Authors - Matteo Marchesini View Article
human	IHC	Appl Immunohistochem Mol Morphol Increased PA2G4 Expression Is an Unfavorable Factor in Nasopharyngeal Carcinoma Authors - Yan Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PA2G4 Polyclonal antibody

PA2G4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 15348-1-AP

WB analysis using 15348-1-AP

WB analysis of rat spleen using 15348-1-AP

IP experiment of Jurkat using 15348-1-AP

IHC staining of human breast cancer using 15348-1-AP

IHC staining of human breast cancer using 15348-1-AP

IF Staining of HepG2 using 15348-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.

Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency

Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells

Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma

Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML

Increased PA2G4 Expression Is an Unfavorable Factor in Nasopharyngeal Carcinoma