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  • Featured Product
  • KD/KO Validated

PA2G4 Monoclonal antibody, PBS Only (Detector)

PA2G4 Monoclonal Antibody for WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G10D9

Cat no : 66055-1-PBS

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PA2G4 Monoclonal antibody (66055-1-Ig)

    WB analysis using 66055-1-Ig (same clone as 66055-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66055-1-Ig (PA2G4 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PA2G4 Monoclonal antibody (66055-1-Ig)

    WB analysis of HEK-293 using 66055-1-Ig (same clone as 66055-1-PBS)

    WB result of PA2G4 antibody (66055-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50029-1

    Sandwich ELISA standard curve of MP50029-1

    Sandwich ELISA standard curve of MP50029-1, PA2G4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66055-2-PBS. Detection antibody: HRP-conjugated 66055-1-PBS. Standard: Ag7850. Range: 1.25-20 ng/mL.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PA2G4 Monoclonal antibody (66055-1-Ig)

    IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PA2G4 Monoclonal antibody (66055-1-Ig)

    IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PA2G4 Monoclonal antibody (66055-1-Ig)

    IF Staining of HeLa using 66055-1-Ig (same clone as 66055-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PA2G4 antibody (66055-1-Ig, Clone: 3G10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66055-1-PBS targets PA2G4 as part of a matched antibody pair:

MP50029-1: 66055-2-PBS capture and 66055-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PA2G4 fusion protein Ag7850 相同性解析による交差性が予測される生物種
Full Name proliferation-associated 2G4, 38kDa
Calculated molecular weight 44 kDa
Observed molecular weight 38-42 kDa, 48 kDa
GenBank accession numberBC001951
Gene symbol PA2G4
Gene ID (NCBI) 5036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.