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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PAAF1 Polyclonal antibody (17650-1-AP)

    WB analysis of Jurkat using 17650-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    WB analysis of mouse brain using 17650-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    WB analysis of human brain using 17650-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PAAF1 Polyclonal antibody (17650-1-AP)

    WB analysis of HEK-293 using 17650-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human testis using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human testis using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PAAF1 Polyclonal antibody (17650-1-AP)

    IF Staining of U2OS using 17650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PAAF1 Polyclonal antibody

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17650-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, human brain tissue, mouse brain tissue
Positive IHC detected inmouse kidney tissue, human brain tissue, human kidney tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17650-1-AP targets PAAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAAF1 fusion protein Ag11892 相同性解析による交差性が予測される生物種
Full Name proteasomal ATPase-associated factor 1
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC028628
Gene Symbol PAAF1
Gene ID (NCBI) 80227
RRIDAB_2878424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRP4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PAAF1 antibody 17650-1-APDownload protocol
IHC protocol for PAAF1 antibody 17650-1-APDownload protocol
IF protocol for PAAF1 antibody 17650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

PMID: 31285951

Authors - Wen-Si Zhao
View Article
humanIHC

Elife

Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes

Authors - Aojia Zhuang
View Article