Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10970-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 10970-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10970-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 10970-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10970-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10970-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10970-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10970-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10970-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 10970-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 10970-1-AP
IP result of anti-PABPC1,PABP (IP:10970-1-AP, 4ug; Detection:10970-1-AP 1:700) with mouse testis tissue lysate 4000ug.
IHC staining of human pancreas using 10970-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 10970-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10970-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.
IHC staining of human testis using 10970-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.
IF Staining of MCF-7 using 10970-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PABPC1,PABP antibody (10970-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

PABPC1,PABP Polyclonal antibody

PABPC1,PABP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10970-1-AP

Publications(39)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

PABP, PABP1, PABPC1, PABPC 1, PABP-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (39)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HCT 116 cells, mouse testis tissue, PC-3 cells, HeLa cells, A549 cells, rat testis tissue, HT-29 cells, MCF-7 cells, NCI-H1299 cells, HCT-116 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human pancreas tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 29 publications below
IHC	See 3 publications below
IF	See 9 publications below
IP	See 5 publications below
CoIP	See 1 publications below
RIP	See 3 publications below

Product Information

10970-1-AP targets PABPC1,PABP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PABPC1,PABP fusion protein Ag1422 相同性解析による交差性が予測される生物種
Full Name	poly(A) binding protein, cytoplasmic 1
Calculated molecular weight	71 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC015958
Gene Symbol	PABPC1
Gene ID (NCBI)	26986
RRID	AB_10596918
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11940
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Protocols

Product Specific Protocols
WB protocol for PABPC1,PABP antibody 10970-1-AP	Download protocol
IHC protocol for PABPC1,PABP antibody 10970-1-AP	Download protocol
IF protocol for PABPC1,PABP antibody 10970-1-AP	Download protocol
IP protocol for PABPC1,PABP antibody 10970-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Brain Behav Immun Transcriptomic and proteomic profiling of bi-partite and tri-partite murine iPSC-derived neurospheroids under steady-state and inflammatory condition Authors - Julia Di Stefano View Article
human	WB	Exp Mol Med The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability Authors - Qianqian Yang View Article
human	WB	Nat Commun An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. Authors - Song Zhu View Article
mouse	WB	Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice Authors - Dingfeng Zou View Article
human	WB	J Exp Med E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer. Authors - Ke Li View Article
human	WB	Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing Authors - Qi Wen Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10970-1-AP

WB analysis using 10970-1-AP

WB analysis using 10970-1-AP

WB analysis using 10970-1-AP

WB analysis of HeLa using 10970-1-AP

WB analysis of mouse testis using 10970-1-AP

WB analysis of PC-3 using 10970-1-AP

WB analysis of mouse testis using 10970-1-AP

WB analysis of HeLa using 10970-1-AP

WB analysis of rat testis using 10970-1-AP

IP experiment of mouse testis using 10970-1-AP

IHC staining of human pancreas using 10970-1-AP

IHC staining of human pancreas using 10970-1-AP

IHC staining of human testis using 10970-1-AP

IHC staining of human testis using 10970-1-AP

IF Staining of MCF-7 using 10970-1-AP

PABPC1,PABP Polyclonal antibody

PABPC1,PABP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptomic and proteomic profiling of bi-partite and tri-partite murine iPSC-derived neurospheroids under steady-state and inflammatory condition

The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing