• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PABPC3 Polyclonal antibody

PABPC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12625-2-AP

Synonyms

PABP 3, PABP3, PABPC3, PABPL3, Poly(A) binding protein 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PABPC3 Polyclonal antibody (12625-2-AP)

    WB analysis using 12625-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12625-2-AP (PABPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    WB analysis of mouse testis using 12625-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12625-2-AP (PABPC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    IP experiment of mouse testis using 12625-2-AP

    IP result of anti-PABPC3 (IP:12625-2-AP, 4ug; Detection:12625-2-AP 1:300) with mouse testis tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    IHC staining of human testis using 12625-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12625-2-AP (PABPC3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    IHC staining of human testis using 12625-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12625-2-AP (PABPC3 antibody) at dilution of 1:50 (under 10x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inrat testis tissue, mouse testis tissue, Jurkat cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12625-2-AP targets PABPC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PABPC3 fusion protein Ag3317 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein, cytoplasmic 3
Calculated molecular weight 631 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC027617
Gene symbol PABPC3
Gene ID (NCBI) 5042
RRIDAB_2156892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PABPC3 antibody 12625-2-APDownload protocol
IHC protocol for PABPC3 antibody 12625-2-APDownload protocol
IP protocol for PABPC3 antibody 12625-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Assist Reprod Genet

The poly(A)-binding protein genes, EPAB, PABPC1, and PABPC3 are differentially expressed in infertile men with non-obstructive azoospermia.

Authors - Saffet Ozturk
View Article