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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using PABPC4 Monoclonal antibody (68187-1-Ig)

    WB analysis of HSC-T6 using 68187-1-Ig (same clone as 68187-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PABPC4 Monoclonal antibody (68187-1-Ig)

    WB analysis using 68187-1-Ig (same clone as 68187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PABPC4 Monoclonal antibody (68187-1-Ig)

    WB analysis of NIH/3T3 using 68187-1-Ig (same clone as 68187-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PABPC4 Monoclonal antibody (68187-1-Ig)

    IHC staining of mouse skeletal muscle using 68187-1-Ig (same clone as 68187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PABPC4 Monoclonal antibody (68187-1-Ig)

    IHC staining of human colon cancer using 68187-1-Ig (same clone as 68187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PABPC4 Monoclonal antibody (68187-1-Ig)

    IHC staining of human colon cancer using 68187-1-Ig (same clone as 68187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PABPC4 Monoclonal antibody (68187-1-Ig)

    IHC staining of mouse skeletal muscle using 68187-1-Ig (same clone as 68187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PABPC4 Monoclonal antibody (68187-1-Ig)

    IF Staining of A431 using 68187-1-Ig (same clone as 68187-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PABPC4 antibody (68187-1-Ig, Clone: 1E6B3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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PABPC4 Monoclonal antibody, PBS Only

PABPC4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68187-1-PBS
Clone No.1E6B3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Activated platelet protein 1, APP 1, APP1, FLJ43938, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68187-1-PBS targets PABPC4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PABPC4 fusion protein Ag6813 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein, cytoplasmic 4 (inducible form)
Calculated molecular weight 70 kDa
Observed molecular weight71 kDa
GenBank accession numberBC065540
Gene Symbol PABPC4
Gene ID (NCBI) 8761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13310
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Poly(A)-binding protein (PABP), a nucleocytoplasmic shuttling protein, has a fundamental role in promoting gene expression by enhancing mRNA translation and stability(PMID: 21940797). Poly(A)-binding protein 4 (PABPC4), one of the homologs of PABP, was initially identified as a human T-cell activation-induced protein. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules(NCBI).