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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PABPN1 Monoclonal antibody (66807-1-Ig)

    WB analysis of HeLa using 66807-1-Ig (same clone as 66807-1-PBS)

    WB result of PABPN1 antibody (66807-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPN1 transfected HeLa cells. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PABPN1 Monoclonal antibody (66807-1-Ig)

    WB analysis using 66807-1-Ig (same clone as 66807-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PABPN1 Monoclonal antibody (66807-1-Ig)

    WB analysis using 66807-1-Ig (same clone as 66807-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PABPN1 Monoclonal antibody (66807-1-Ig)

    IP experiment of Jurkat using 66807-1-Ig (same clone as 66807-1-PBS)

    IP result of anti-PABPN1 (IP:66807-1-Ig, 5ug; Detection:66807-1-Ig 1:600) with Jurkat cells lysate 1680 ug. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of human colon using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of mouse heart using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of mouse heart using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of rat heart using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of human colon using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of human colon cancer using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of human colon cancer using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using PABPN1 Monoclonal antibody (66807-1-Ig)

    IHC staining of rat heart using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PABPN1 Monoclonal antibody (66807-1-Ig)

    IF Staining of MCF-7 using 66807-1-Ig (same clone as 66807-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PABPN1 antibody (66807-1-Ig, Clone: 3C12E12 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PABPN1 Monoclonal antibody, PBS Only

PABPN1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66807-1-PBS
Clone No.3C12E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66807-1-PBS targets PABPN1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PABPN1 fusion protein Ag27470 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein, nuclear 1
Calculated molecular weight 33 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC010939
Gene Symbol PABPN1
Gene ID (NCBI) 8106
RRIDAB_2882150
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86U42
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).