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Validation Data Gallery

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  • Western Blot (WB) analysis of HCT 116 cells using PACS2 Polyclonal antibody (30172-1-AP)

    WB analysis of HCT 116 using 30172-1-AP

    WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells.

  • Western Blot (WB) analysis of various lysates using PACS2 Polyclonal antibody (30172-1-AP)

    WB analysis using 30172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PACS2 Polyclonal antibody (30172-1-AP)

    WB analysis using 30172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HCT 116 cells using PACS2 Polyclonal antibody (30172-1-AP)

    IP experiment of HCT 116 using 30172-1-AP

    IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using PACS2 Polyclonal antibody (30172-1-AP)

    IF Staining of HCT 116 using 30172-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

PACS2 Polyclonal antibody

PACS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 30172-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

FLJ25488, KIAA0602, PACS 2, PACS1 like protein, PACS1L, PACS2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, NIH/3T3 cells, HuH-7 cells
Positive IP detected inHCT 116 cells
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30172-1-AP targets PACS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PACS2 fusion protein Ag32902 相同性解析による交差性が予測される生物種
Full Name phosphofurin acidic cluster sorting protein 2
Calculated molecular weight98kd
Observed molecular weight100-130 kDa
GenBank accession numberNM_001100913
Gene Symbol PACS2
Gene ID (NCBI) 23241
RRIDAB_3086254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

Protocols

Product Specific Protocols
WB protocol for PACS2 antibody 30172-1-APDownload protocol
IF protocol for PACS2 antibody 30172-1-APDownload protocol
IP protocol for PACS2 antibody 30172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols