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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PACSIN3 Polyclonal antibody

PACSIN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10639-1-AP

Synonyms

SH3 domain-containing protein 6511, Protein kinase C and casein kinase substrate in neurons protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of HeLa using 10639-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of rat brain using 10639-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of mouse liver using 10639-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of mouse heart using 10639-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of mouse skeletal muscle using 10639-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of mouse lung using 10639-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    IHC staining of human breast cancer using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    IHC staining of human heart using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    IHC staining of human heart using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PACSIN3 Polyclonal antibody (10639-1-AP)

    IF Staining of HeLa using 10639-1-AP

    Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10639-1-AP targets PACSIN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PACSIN3 fusion protein Ag1006 相同性解析による交差性が予測される生物種
Full Name protein kinase C and casein kinase substrate in neurons 3
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC007914
Gene symbol PACSIN3
Gene ID (NCBI) 29763
RRIDAB_2159453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PACSIN3 antibody 10639-1-APDownload protocol
IHC protocol for PACSIN3 antibody 10639-1-APDownload protocol
IF protocol for PACSIN3 antibody 10639-1-APDownload protocol
FC protocol for PACSIN3 antibody 10639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

Biallelic truncating variants in PACSIN3 cause childhood-onset myopathy with hyperCKaemia

Authors - Felix Distelmaier
View Article