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KD/KO Validated

PADI2 Polyclonal antibody

Name: PADI2 Polyclonal antibody
Brand: Proteintech
SKU: 12110-1-AP

PADI2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12110-1-AP

Synonyms

KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICC

WB analysis using 12110-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12110-1-AP
WB result of PADI2 antibody (12110-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.
WB analysis of MCF-7 using 12110-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12110-1-AP
IP result of anti-PADI2 (IP:12110-1-AP, 3ug; Detection:12110-1-AP 1:300) with mouse brain tissue lysate 3600ug.
IHC staining of human breast cancer using 12110-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12110-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human breast cancer using 12110-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human breast cancer using 12110-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human breast cancer using 12110-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of BxPC-3 using 12110-1-AP
Immunofluorescent analysis of BxPC-3 cells using 12110-1-AP (PADI2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 12110-1-AP
Immunofluorescent analysis of HeLa cells using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12110-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human breast cancer tissue
Positive IF/ICC detected in	BxPC-3 cells, human breast cancer tissue, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 35 publications below
IHC	See 12 publications below
IF	See 19 publications below
FC	See 2 publications below
CoIP	See 1 publications below

Product Information

12110-1-AP targets PADI2 in WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, pig, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PADI2 fusion protein Ag2755 相同性解析による交差性が予測される生物種
Full Name	peptidyl arginine deiminase, type II
Calculated molecular weight	665 aa, 75 kDa
Observed molecular weight	70-75 kDa, 50 kDa
GenBank accession number	BC009701
Gene symbol	PADI2
Gene ID (NCBI)	11240
RRID	AB_2159475
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Protocols

Product Specific Protocols
WB protocol for PADI2 antibody 12110-1-AP	Download protocol
IHC protocol for PADI2 antibody 12110-1-AP	Download protocol
IF protocol for PADI2 antibody 12110-1-AP	Download protocol
IP protocol for PADI2 antibody 12110-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa. Authors - Angel S Byrd View Article
human	WB	Mol Cell Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Authors - Priyanka Sharma KD Validated View Article
mouse	WB,FC	J Immunother Cancer PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy. Authors - Ruhul H Choudhury KO Validated View Article
mouse	WB,IF	Angiogenesis Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes. Authors - Jing Bai KD Validated View Article
human	WB	Proc Natl Acad Sci U S A Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation. Authors - Zhang Xuesen X View Article
human	WB	Diabetes Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes. Authors - Meghan L Marre View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis using 12110-1-AP

WB analysis of MCF-7 using 12110-1-AP

WB analysis of MCF-7 using 12110-1-AP

IP experiment of mouse brain using 12110-1-AP

IHC staining of human breast cancer using 12110-1-AP

IHC staining of human breast cancer using 12110-1-AP

IF Staining of human breast cancer using 12110-1-AP

IF Staining of human breast cancer using 12110-1-AP

IF Staining of human breast cancer using 12110-1-AP

IF Staining of BxPC-3 using 12110-1-AP

IF Staining of HeLa using 12110-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.

Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription.

PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.

Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.

Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation.

Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes.

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