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PADI2 Polyclonal antibody
PADI2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay
Conjugate
Unconjugated
Cat no : 12110-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, MCF-7 cells, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
Positive IF/ICC detected in | BxPC-3 cells, human breast cancer tissue, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 12 publications below |
WB | See 35 publications below |
IHC | See 12 publications below |
IF | See 19 publications below |
FC | See 2 publications below |
CoIP | See 1 publications below |
Product Information
12110-1-AP targets PADI2 in WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse, pig, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PADI2 fusion protein Ag2755 相同性解析による交差性が予測される生物種 |
Full Name | peptidyl arginine deiminase, type II |
Calculated molecular weight | 665 aa, 75 kDa |
Observed molecular weight | 70-75 kDa, 50 kDa |
GenBank accession number | BC009701 |
Gene symbol | PADI2 |
Gene ID (NCBI) | 11240 |
RRID | AB_2159475 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for PADI2 antibody 12110-1-AP | Download protocol |
IHC protocol for PADI2 antibody 12110-1-AP | Download protocol |
IF protocol for PADI2 antibody 12110-1-AP | Download protocol |
IP protocol for PADI2 antibody 12110-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Transl Med Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa. | ||
Mol Cell Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription.
| ||
J Immunother Cancer PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.
| ||
Angiogenesis Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.
| ||
Proc Natl Acad Sci U S A Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation. | ||
Diabetes Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes. |