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  • Featured Product
  • KD/KO Validated

PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay

Conjugate

Unconjugated

Cat no : 12110-1-AP

Synonyms

PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PADI2 Polyclonal antibody (12110-1-AP)

    WB analysis using 12110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PADI2 Polyclonal antibody (12110-1-AP)

    WB analysis of MCF-7 using 12110-1-AP

    WB result of PADI2 antibody (12110-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.

  • Western Blot (WB) analysis of MCF-7 cells using PADI2 Polyclonal antibody (12110-1-AP)

    WB analysis of MCF-7 using 12110-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PADI2 Polyclonal antibody (12110-1-AP)

    IP experiment of mouse brain using 12110-1-AP

    IP result of anti-PADI2 (IP:12110-1-AP, 3ug; Detection:12110-1-AP 1:300) with mouse brain tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PADI2 Polyclonal antibody (12110-1-AP)

    IHC staining of human breast cancer using 12110-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PADI2 Polyclonal antibody (12110-1-AP)

    IHC staining of human breast cancer using 12110-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using PADI2 Polyclonal antibody (12110-1-AP)

    IF Staining of human breast cancer using 12110-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PADI2 Polyclonal antibody (12110-1-AP)

    IF Staining of HeLa using 12110-1-AP

    Immunofluorescent analysis of HeLa cells using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using PADI2 Polyclonal antibody (12110-1-AP)

    IF Staining of BxPC-3 using 12110-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 12110-1-AP (PADI2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inBxPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12110-1-AP targets PADI2 in WB, IHC, IF/ICC, IF-P, IP, COIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PADI2 fusion protein Ag2755 相同性解析による交差性が予測される生物種
Full Name peptidyl arginine deiminase, type II
Calculated molecular weight 665 aa, 75 kDa
Observed molecular weight 70-75 kDa, 50 kDa
GenBank accession numberBC009701
Gene symbol PADI2
Gene ID (NCBI) 11240
RRIDAB_2159475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Protocols

Product Specific Protocols
WB protocol for PADI2 antibody 12110-1-APDownload protocol
IHC protocol for PADI2 antibody 12110-1-APDownload protocol
IF protocol for PADI2 antibody 12110-1-APDownload protocol
IP protocol for PADI2 antibody 12110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Sci Transl Med

Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.

Authors - Angel S Byrd
View Article
humanWB

Mol Cell

Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription.

Authors - Priyanka Sharma
  • KD Validated
View Article
mouseWB,FC

J Immunother Cancer

PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.

Authors - Ruhul H Choudhury
  • KO Validated
View Article
mouseWB,IF

Angiogenesis

Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.

Authors - Jing Bai
  • KD Validated
View Article
humanWB

Proc Natl Acad Sci U S A

Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation.

Authors - Zhang Xuesen X
View Article
humanWB

Diabetes

Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes.

Authors - Meghan L Marre
View Article