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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29169-1-AP

Synonyms

KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PADI2 Polyclonal antibody (29169-1-AP)

    WB analysis using 29169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29169-1-AP (PADI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PADI2 Polyclonal antibody (29169-1-AP)

    IHC staining of human kidney using 29169-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29169-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PADI2 Polyclonal antibody (29169-1-AP)

    IHC staining of human kidney using 29169-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29169-1-AP (PADI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29169-1-AP targets PADI2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PADI2 fusion protein Ag29042 相同性解析による交差性が予測される生物種
Full Name peptidyl arginine deiminase, type II
Calculated molecular weight 665 aa, 75 kDa
Observed molecular weight75 kDa
GenBank accession numberBC009701
Gene symbol PADI2
Gene ID (NCBI) 11240
RRIDAB_2918243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Protocols

Product Specific Protocols
WB protocol for PADI2 antibody 29169-1-APDownload protocol
IHC protocol for PADI2 antibody 29169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols