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PADI2 Monoclonal antibody
PADI2 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, rabbit, pig
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2D8C12
Cat no : 66386-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human skeletal muscle tissue, HeLa cells, MCF-7 cells, pig brain tissue, rat brain tissue, mouse brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
66386-1-Ig targets PADI2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.
Tested Reactivity | human, mouse, rat, rabbit, pig |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | PADI2 fusion protein Ag17612 相同性解析による交差性が予測される生物種 |
Full Name | peptidyl arginine deiminase, type II |
Calculated molecular weight | 665 aa, 75 kDa |
Observed molecular weight | 75 kDa |
GenBank accession number | BC009701 |
Gene symbol | PADI2 |
Gene ID (NCBI) | 11240 |
RRID | AB_2881762 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for PADI2 antibody 66386-1-Ig | Download protocol |
IHC protocol for PADI2 antibody 66386-1-Ig | Download protocol |
IF protocol for PADI2 antibody 66386-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination
| ||
Cell Mol Life Sci ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.
| ||
Acta Pharm Sin B Cholinergic dysfunction-induced insufficient activation of alpha7 nicotinic acetylcholine receptor drives the development of rheumatoid arthritis through promoting protein citrullination via the SP3/PAD4 pathway |