Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29304-1-AP targets PAFAH in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAFAH fusion protein Ag30947 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 29-30 kDa, 45-55 kDa |
| GenBank accession number | BC038452 |
| Gene Symbol | PLA2G7 |
| Gene ID (NCBI) | 7941 |
| RRID | AB_2918276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Platelet-activating factor acetylhydrolase (PAFAH) Ib consists of homo- or heterodimers of two PLA2 subunits α1 and α2, and a dimer of a third subunit, LIS1. PAFAH Ib was first discovered by its ability to hydrolyze the extracellular signaling lipid, PAF, which plays a role in platelet activation and inflammation. LIS1, the causative agent of the fatal brain disorder Miller-Dieker lissencephaly, functions independently of the α subunits to regulate the activity of cytoplasmic dynein. LIS1 is a highly conserved protein involved in dynein-mediated processes including nuclear and neuronal migration, centrosomal function, and mitotic spindle orientation. (PMID: 20624900, PMID: 23262398)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAFAH antibody 29304-1-AP | Download protocol |
| IHC protocol for PAFAH antibody 29304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |