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  • Featured Product
  • KD/KO Validated

PAFAH1B2 Polyclonal antibody

PAFAH1B2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14729-1-AP

Synonyms

PAF AH 30 kDa subunit, PAF AH subunit beta, PAFAH subunit beta, PAFAH1B2, PAFAHB

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using PAFAH1B2 Polyclonal antibody (14729-1-AP)

    WB analysis of human testis using 14729-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14729-1-AP (PAFAH1B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using PAFAH1B2 Polyclonal antibody (14729-1-AP)

    WB analysis of mouse small intestine using 14729-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14729-1-AP (PAFAH1B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PAFAH1B2 Polyclonal antibody (14729-1-AP)

    WB analysis of human testis using 14729-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14729-1-AP (PAFAH1B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PAFAH1B2 Polyclonal antibody (14729-1-AP)

    IP experiment of mouse testis using 14729-1-AP

    IP result of anti-PAFAH1B2 (IP:14729-1-AP, 4ug; Detection:14729-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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Tested Applications

Positive WB detected inhuman testis tissue, mouse small intestine tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14729-1-AP targets PAFAH1B2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAFAH1B2 fusion protein Ag6489 相同性解析による交差性が予測される生物種
Full Name platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000398
Gene symbol PAFAH1B2
Gene ID (NCBI) 5049
RRIDAB_2159909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PAFAH1B2 antibody 14729-1-APDownload protocol
IP protocol for PAFAH1B2 antibody 14729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Cell Int

Platelet-activating factor acetyl hydrolase IB2 dysregulated cell proliferation in ovarian cancer.

Authors - YingYing He
  • KD Validated
View Article
mouseWB

Biol Pharm Bull

A Complex of Type I Platelet-Activating Factor Acetylhydrolase (PAF-AH) Catalytic Subunits Switches from α1/α2 Heterodimer to α2/α2 Homodimer during Adipocyte Differentiation of 3T3-L1 Cells

Authors - Yasuhiro Nakamura
View Article