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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAG1 Polyclonal antibody

PAG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25029-1-AP

Synonyms

Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, PAG, Csk-binding protein, Csk binding protein, CBP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PAG1 Polyclonal antibody (25029-1-AP)

    WB analysis of mouse brain using 25029-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25029-1-AP (PAG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PAG1 Polyclonal antibody (25029-1-AP)

    IHC staining of human ovary cancer using 25029-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25029-1-AP (PAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25029-1-AP targets PAG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAG1 fusion protein Ag13393 相同性解析による交差性が予測される生物種
Full Name phosphoprotein associated with glycosphingolipid microdomains 1
Calculated molecular weight 432 aa, 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC112159
Gene symbol PAG1
Gene ID (NCBI) 55824
RRIDAB_2879858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAG1, also named as CBP and PAG, promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It is involved in cell adhesion signaling. PAG1 may play an important role in inhibiting the proliferation, invasion and metastasis of the cancer cells. PAG1 is a membrane protein with phosphor modification. The MW of PAG1 migrates to 50-65 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for PAG1 antibody 25029-1-APDownload protocol
IHC protocol for PAG1 antibody 25029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols