PAIP2 Polyclonal antibody

Name: PAIP2 Polyclonal antibody
Brand: Proteintech
SKU: 15583-1-AP

PAIP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15583-1-AP

Synonyms

PAIP2A, PAIP-2, PAIP 2, PABP-interacting protein 2, PABP interacting protein 2

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of A431 using 15583-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15583-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15583-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15583-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15583-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 15583-1-AP
IP result of anti-PAIP2 (IP:15583-1-AP, 4ug; Detection:15583-1-AP 1:500) with A431 cells lysate 1200ug.
IF Staining of HeLa using 15583-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15583-1-AP (PAIP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 15583-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, Jurkat cells, mouse lung tissue
Positive IP detected in	A431 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

15583-1-AP targets PAIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PAIP2 fusion protein Ag7119 相同性解析による交差性が予測される生物種
Full Name	poly(A) binding protein interacting protein 2
Calculated molecular weight	15 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC001716
Gene symbol	PAIP2
Gene ID (NCBI)	51247
RRID	AB_10950891
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PAIP2 antibody 15583-1-AP	Download protocol
IF protocol for PAIP2 antibody 15583-1-AP	Download protocol
IP protocol for PAIP2 antibody 15583-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	J Neurosci Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity. Authors - Chenghai Dong View Article
human	WB	Front Genet PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration Authors - Chenyu Wang View Article
mouse	IP	Pharmacol Res Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation Authors - Yuan Gao View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAIP2 Polyclonal antibody

PAIP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A431 using 15583-1-AP

WB analysis of HeLa using 15583-1-AP

WB analysis of Jurkat using 15583-1-AP

WB analysis of Jurkat using 15583-1-AP

WB analysis of mouse lung using 15583-1-AP

IP experiment of A431 using 15583-1-AP

IF Staining of HeLa using 15583-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity.

PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration

Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation