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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PAIP2 Polyclonal antibody (15583-1-AP)

    WB analysis of A431 using 15583-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PAIP2 Polyclonal antibody (15583-1-AP)

    WB analysis of HeLa using 15583-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PAIP2 Polyclonal antibody (15583-1-AP)

    WB analysis of Jurkat using 15583-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PAIP2 Polyclonal antibody (15583-1-AP)

    WB analysis of Jurkat using 15583-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using PAIP2 Polyclonal antibody (15583-1-AP)

    WB analysis of mouse lung using 15583-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using PAIP2 Polyclonal antibody (15583-1-AP)

    IP experiment of A431 using 15583-1-AP

    IP result of anti-PAIP2 (IP:15583-1-AP, 4ug; Detection:15583-1-AP 1:500) with A431 cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PAIP2 Polyclonal antibody (15583-1-AP)

    IF Staining of HeLa using 15583-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15583-1-AP (PAIP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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PAIP2 Polyclonal antibody

PAIP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15583-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PAIP2A, PAIP-2, PAIP 2, PABP-interacting protein 2, PABP interacting protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, mouse lung tissue
Positive IP detected inA431 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15583-1-AP targets PAIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAIP2 fusion protein Ag7119 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein interacting protein 2
Calculated molecular weight 15 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC001716
Gene Symbol PAIP2
Gene ID (NCBI) 51247
RRIDAB_10950891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPZ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PAIP2 antibody 15583-1-APDownload protocol
IF protocol for PAIP2 antibody 15583-1-APDownload protocol
IP protocol for PAIP2 antibody 15583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Neurosci

Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity.

Authors - Chenghai Dong
View Article
humanWB

Front Genet

PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration

Authors - Chenyu Wang
View Article
mouseIP

Pharmacol Res

Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation

Authors - Yuan Gao
View Article