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KD/KO Validated

PAK1 Polyclonal antibody

Name: PAK1 Polyclonal antibody
Brand: Proteintech
SKU: 21401-1-AP

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21401-1-AP

Synonyms

p65 PAK, p21-activated kinase 1, p21 activated kinase 1, EC:2.7.11.1, Alpha-PAK

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 21401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 21401-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21401-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21401-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21401-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21401-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21401-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 21401-1-AP
IP result of anti-PAK1 (IP:21401-1-AP, 4ug; Detection:21401-1-AP 1:1000) with K-562 cells lysate 3200ug.
IHC staining of mouse brain using 21401-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 21401-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21401-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21401-1-AP (PAK1 Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 21401-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21401-1-AP (PAK1 Antibody) at dilution of 1:50.
IF Staining of C6 using 21401-1-AP
Immunofluorescent analysis of C6 cells using 21401-1-AP (PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of C6 using 21401-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PAK1 antibody (21401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 21401-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C6 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	C6 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 17 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

21401-1-AP targets PAK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PAK1 fusion protein Ag16102 相同性解析による交差性が予測される生物種
Full Name	p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight	553 aa, 62 kDa
Observed molecular weight	61-65 kDa
GenBank accession number	BC109299
Gene symbol	PAK1
Gene ID (NCBI)	5058
RRID	AB_11232232
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

Protocols

Product Specific Protocols
WB protocol for PAK1 antibody 21401-1-AP	Download protocol
IHC protocol for PAK1 antibody 21401-1-AP	Download protocol
IF protocol for PAK1 antibody 21401-1-AP	Download protocol
IP protocol for PAK1 antibody 21401-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res DOCK7 protects against replication stress by promoting RPA stability on chromatin. Authors - Ming Gao View Article
mouse	WB	ACS Appl Mater Interfaces Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function. Authors - Yi Li View Article
mouse	IF,WB	Theranostics Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development. Authors - Min Zhang View Article
rat	WB	Acta Pharmacol Sin Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway. Authors - Cheng-di Liu View Article
chicken	WB	Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization. Authors - Beibei Zhang View Article
mouse	WB	Sci Rep Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module. Authors - Dongli Liang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAK1 Polyclonal antibody

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 21401-1-AP

WB analysis using 21401-1-AP

WB analysis of HeLa using 21401-1-AP

WB analysis of K-562 using 21401-1-AP

WB analysis of NIH/3T3 using 21401-1-AP

WB analysis of MCF-7 using 21401-1-AP

WB analysis of Jurkat using 21401-1-AP

IP experiment of K-562 using 21401-1-AP

IHC staining of mouse brain using 21401-1-AP

IHC staining of mouse brain using 21401-1-AP

IHC staining of human breast cancer using 21401-1-AP

IHC staining of human breast cancer using 21401-1-AP

IF Staining of C6 using 21401-1-AP

IF Staining of C6 using 21401-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway.

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.