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  • Featured Product
  • KD/KO Validated

PAK1 Polyclonal antibody

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 51137-1-AP

Synonyms

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis using 51137-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis of HEK-293 using 51137-1-AP

    WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of K-562 cells using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis of K-562 using 51137-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis of A431 using 51137-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PAK1 Polyclonal antibody (51137-1-AP)

    IHC staining of human stomach cancer using 51137-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PAK1 Polyclonal antibody (51137-1-AP)

    IHC staining of human stomach cancer using 51137-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PAK1 Polyclonal antibody (51137-1-AP)

    IF Staining of HeLa using 51137-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA431 cells, K-562 cells, HEK-293 cells, C6 cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51137-1-AP targets PAK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight 553 aa, 62 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC109299
Gene symbol PAK1
Gene ID (NCBI) 5058
RRIDAB_2283523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

Protocols

Product Specific Protocols
WB protocol for PAK1 antibody 51137-1-APDownload protocol
IHC protocol for PAK1 antibody 51137-1-APDownload protocol
IF protocol for PAK1 antibody 51137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Pathol Res Pract

Clinicopathological significance and the associated signaling pathway of p21-activated kinase 1 (PAK1) in colorectal cancer

Authors - Jung-Soo Pyo
View Article
humanWB

Commun Biol

Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia

Authors - Janet P Raja Xavier
View Article