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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse brain tissue using PAK4/6/7 Polyclonal antibody (12460-1-AP)

    IP experiment of mouse brain using 12460-1-AP (same clone as 12460-1-PBS)

    IP result of anti-PAK4/6/7 (IP:12460-1-AP, 4ug; Detection:12460-1-AP 1:300) with mouse brain tissue lysate 5200ug. This data was developed using the same antibody clone with 12460-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAK4/6/7 Polyclonal antibody (12460-1-AP)

    IHC staining of mouse brain using 12460-1-AP (same clone as 12460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12460-1-AP (PAK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12460-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAK4/6/7 Polyclonal antibody (12460-1-AP)

    IHC staining of mouse brain using 12460-1-AP (same clone as 12460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12460-1-AP (PAK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12460-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12460-1-PBS targets PAK4/6/7 in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAK4/6/7 fusion protein Ag3127 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
RRIDAB_2877858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P286
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. The antibody can recognize PAK4/6/7. PAK4/6/7 belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It acts on a variety of targets.