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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK4 Monoclonal antibody (68298-1-Ig)

    WB analysis using 68298-1-Ig (same clone as 68298-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68298-1-Ig (PAK4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PAK4 Monoclonal antibody (68298-1-Ig)

    IF Staining of HeLa using 68298-1-Ig (same clone as 68298-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PAK4 antibody (68298-1-Ig, Clone: 2G6A3 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

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PAK4 Monoclonal antibody, PBS Only

PAK4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68298-1-PBS
Clone No.2G6A3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68298-1-PBS targets PAK4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAK4 fusion protein Ag6364 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 4
Calculated molecular weight 64 kDa
Observed molecular weight64 kDa
GenBank accession numberBC025282
Gene Symbol PAK4
Gene ID (NCBI) 10298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96013
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).