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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK4 Recombinant antibody (83761-5-RR)

    WB analysis using 83761-5-RR (same clone as 83761-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83761-5-RR (PAK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83761-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PAK4 Recombinant antibody (83761-5-RR)

    FC experiment of MCF-7 using 83761-5-RR (same clone as 83761-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PAK4 Recombinant antibody (83761-5-RR, Clone:240686D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83761-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83761-5-RR

    Affinity and Kinetic Characterization of 83761-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83761-5-RR against Human PAK4 were performed. The affinity constant is below 1 pM.

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PAK4 Recombinant antibody, PBS Only

PAK4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83761-5-PBS
Clone No.240686D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

PAK 4, p21-activated kinase 4, p21 activated kinase 4, KIAA1142, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83761-5-PBS targets PAK4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAK4 fusion protein Ag5981 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 4
Calculated molecular weight 64 kDa
Observed molecular weight64 kDa
GenBank accession numberBC025282
Gene Symbol PAK4
Gene ID (NCBI) 10298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO96013
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).