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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PAK6 Monoclonal antibody (67562-1-Ig)

    WB analysis of K-562 using 67562-1-Ig (same clone as 67562-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67562-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PAK6 Monoclonal antibody (67562-1-Ig)

    WB analysis of HEK-293 using 67562-1-Ig (same clone as 67562-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67562-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PAK6 Monoclonal antibody (67562-1-Ig)

    IF Staining of HEK-293 using 67562-1-Ig (same clone as 67562-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PAK6 antibody (67562-1-Ig, Clone: 3G8C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67562-1-PBS in a different storage buffer formulation.

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PAK6 Monoclonal antibody, PBS Only

PAK6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67562-1-PBS
Clone No.3G8C1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67562-1-PBS targets PAK6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAK6 fusion protein Ag27692 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 6
Calculated molecular weight 681 aa, 75 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC035596
Gene Symbol PAK6
Gene ID (NCBI) 56924
RRIDAB_2882776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQU5
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).