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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK7 Polyclonal antibody (32085-1-AP)

    WB analysis using 32085-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32085-1-AP (PAK7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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PAK7 Polyclonal antibody

PAK7 Polyclonal Antibody for WB, ELISA
Cat No. 32085-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

PAK4/6/7, PAK 7, PAK 5, p21-activated kinase 7, p21-activated kinase 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32085-1-AP targets PAK7 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAK7 fusion protein Ag29190 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight80 kDa
GenBank accession numberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
RRIDAB_3670187
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).

Protocols

Product Specific Protocols
WB protocol for PAK7 antibody 32085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols