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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAK7 Monoclonal antibody

PAK7 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H3B6

Cat no : 66961-1-Ig

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PAK7 Monoclonal antibody (66961-1-Ig)

    WB analysis of mouse brain using 66961-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using PAK7 Monoclonal antibody (66961-1-Ig)

    WB analysis of pig brain using 66961-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PAK7 Monoclonal antibody (66961-1-Ig)

    WB analysis of rat brain using 66961-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PAK7 Monoclonal antibody (66961-1-Ig)

    IF Staining of Neuro-2a using 66961-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PAK7 Monoclonal antibody (66961-1-Ig)

    IF Staining of U-251 using 66961-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IF/ICC detected inNeuro-2a cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66961-1-Ig targets PAK7 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAK7 fusion protein Ag27299 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC024179
Gene symbol PAK7
Gene ID (NCBI) 57144
RRIDAB_2882284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

Protocols

Product Specific Protocols
WB protocol for PAK7 antibody 66961-1-IgDownload protocol
IF protocol for PAK7 antibody 66961-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols