Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue, pig heart tissue, rat heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66601-1-Ig targets Palladin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, pig samples.
| Tested Reactivity | Human, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Palladin fusion protein Ag1288 相同性解析による交差性が予測される生物種 |
| Full Name | palladin, cytoskeletal associated protein |
| Calculated molecular weight | 1383 aa, 151 kDa |
| Observed molecular weight | 151 kDa |
| GenBank accession number | BC013867 |
| Gene Symbol | palladin |
| Gene ID (NCBI) | 23022 |
| RRID | AB_2881961 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WX93 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Palladin antibody 66601-1-Ig | Download protocol |
| IHC protocol for Palladin antibody 66601-1-Ig | Download protocol |
| IF protocol for Palladin antibody 66601-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Med Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling |