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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Palladin-Specific Polyclonal antibody (16179-1-AP)

    WB analysis of HeLa using 16179-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16179-1-AP (Palladin-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Palladin-Specific Polyclonal antibody (16179-1-AP)

    IP experiment of HeLa using 16179-1-AP

    IP result of anti-Palladin-Specific (IP:16179-1-AP, 4ug; Detection:16179-1-AP 1:200) with HeLa cells lysate 1500ug.

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Palladin-Specific Polyclonal antibody

Palladin-Specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 16179-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16179-1-AP targets Palladin-Specific in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name palladin, cytoskeletal associated protein
Calculated molecular weight 151 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_016081
Gene Symbol palladin
Gene ID (NCBI) 23022
RRIDAB_10695626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WX93
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kDa, 140 kDa, and 65 kDa isoforms of this protein.

Protocols

Product Specific Protocols
WB protocol for Palladin-Specific antibody 16179-1-APDownload protocol
IP protocol for Palladin-Specific antibody 16179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols