- Featured Product
- KD/KO Validated
PALMD Polyclonal antibody
PALMD Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16531-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue |
| Positive IP detected in | mouse heart tissue, |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
16531-1-AP targets PALMD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PALMD fusion protein Ag9796 相同性解析による交差性が予測される生物種 |
| Full Name | palmdelphin |
| Calculated molecular weight | 551 aa, 63 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC012570 |
| Gene symbol | PALMD |
| Gene ID (NCBI) | 54873 |
| RRID | AB_2236613 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PALMD antibody 16531-1-AP | Download protocol |
| IHC protocol for PALMD antibody 16531-1-AP | Download protocol |
| IP protocol for PALMD antibody 16531-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD. | ||
Cell Death Dis Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage.
| ||
Front Oncol Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.
| ||
Cell Rep Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease |
