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- KD/KO Validated
PAN2 Polyclonal antibody
PAN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16427-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, HepG2 cells |
| Positive IHC detected in | mouse heart tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
16427-1-AP targets PAN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAN2 fusion protein Ag9670 相同性解析による交差性が予測される生物種 |
| Full Name | PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) |
| Calculated molecular weight | 1198 aa, 135 kDa |
| Observed molecular weight | 135 kDa |
| GenBank accession number | BC024043 |
| Gene symbol | PAN2 |
| Gene ID (NCBI) | 9924 |
| RRID | AB_1851434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAN2 antibody 16427-1-AP | Download protocol |
| IHC protocol for PAN2 antibody 16427-1-AP | Download protocol |
| IF protocol for PAN2 antibody 16427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.
| ||
Nat Struct Mol Biol An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay. | ||
Neurobiol Dis Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes. | ||
Biol Reprod Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation. | ||
PLoS One Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA. | ||
Cells Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). |
