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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAN3 Polyclonal antibody (25655-1-AP)

    WB analysis using 25655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25655-1-AP (PAN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PAN3 Polyclonal antibody (25655-1-AP)

    IHC staining of human ovary cancer using 25655-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25655-1-AP (PAN3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PAN3 Polyclonal antibody

PAN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25655-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Poly(A)-nuclease deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, PAN deadenylation complex subunit 3, PAB1P-dependent poly(A)-specific ribonuclease, hPan3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25655-1-AP targets PAN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAN3 fusion protein Ag22559 相同性解析による交差性が予測される生物種
Full Name PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
Calculated molecular weight 887 aa, 96 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC128180
Gene Symbol PAN3
Gene ID (NCBI) 255967
RRIDAB_3669491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ58A45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform2.

Protocols

Product Specific Protocols
WB protocol for PAN3 antibody 25655-1-APDownload protocol
IHC protocol for PAN3 antibody 25655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols