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  • Featured Product
  • KD/KO Validated

PANX2 Polyclonal antibody

PANX2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26604-1-AP

Synonyms

hPANX2, pannexin 2, PANX2, PX2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PANX2 Polyclonal antibody (26604-1-AP)

    WB analysis of mouse lung using 26604-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PANX2 Polyclonal antibody (26604-1-AP)

    WB analysis of PC-3 using 26604-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using PANX2 Polyclonal antibody (26604-1-AP)

    WB analysis of mouse spleen using 26604-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PANX2 Polyclonal antibody (26604-1-AP)

    IHC staining of human skeletal muscle using 26604-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PANX2 Polyclonal antibody (26604-1-AP)

    IHC staining of human skeletal muscle using 26604-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PANX2 Polyclonal antibody (26604-1-AP)

    IHC staining of human kidney using 26604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PANX2 Polyclonal antibody (26604-1-AP)

    IHC staining of human kidney using 26604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse spleen tissue, PC-3 cells
Positive IHC detected inhuman skeletal muscle tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26604-1-AP is PANX2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PANX2 fusion protein Ag24256 相同性解析による交差性が予測される生物種
Full Name pannexin 2
Calculated molecular weight 677 aa, 74 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC101023
Gene symbol PANX2
Gene ID (NCBI) 56666
RRIDAB_2880572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pannexins (Panx) are proteins homologous to the invertebrate gap junction proteins called innexins (Inx) and are traditionally described as transmembrane channels. Three distinct Panx paralogs (Panx1, Panx2 and Panx3) have been identified in vertebrates. Previous reports on Panx expression and functionality are focused primarily on Panx1 and Panx3 proteins. The exact function of Panx2 remains elusive. Catalog#26604-1-AP specially recognizes the 70 kDa Panx2.

Protocols

Product Specific Protocols
WB protocol for PANX2 antibody 26604-1-APDownload protocol
IHC protocol for PANX2 antibody 26604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nan Fang Yi Ke Da Xue Xue Bao

[Down-regulation of pannexin 2 channel enhances cisplatin-induced apoptosis in testicular cancer I-10 cells].

Authors - Yanxue Yao
  • KD Validated
View Article