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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAPOLG Polyclonal antibody

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24284-1-AP

Synonyms

PAP gamma, Neo-poly(A) polymerase, Neo-PAP, NeoPAP, Neo PAP

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PAPOLG Polyclonal antibody (24284-1-AP)

    WB analysis of HeLa using 24284-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PAPOLG Polyclonal antibody (24284-1-AP)

    WB analysis of MCF-7 using 24284-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PAPOLG Polyclonal antibody (24284-1-AP)

    WB analysis of MCF-7 using 24284-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PAPOLG Polyclonal antibody (24284-1-AP)

    IP experiment of HeLa using 24284-1-AP

    IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using PAPOLG Polyclonal antibody (24284-1-AP)

    IHC staining of human testis using 24284-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PAPOLG Polyclonal antibody (24284-1-AP)

    IHC staining of human testis using 24284-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PAPOLG Polyclonal antibody (24284-1-AP)

    IF Staining of HepG2 using 24284-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24284-1-AP targets PAPOLG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAPOLG fusion protein Ag16208 相同性解析による交差性が予測される生物種
Full Name poly(A) polymerase gamma
Calculated molecular weight 736 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC111701
Gene symbol PAPOLG
Gene ID (NCBI) 64895
RRIDAB_2879471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

Protocols

Product Specific Protocols
WB protocol for PAPOLG antibody 24284-1-APDownload protocol
IHC protocol for PAPOLG antibody 24284-1-APDownload protocol
IF protocol for PAPOLG antibody 24284-1-APDownload protocol
IP protocol for PAPOLG antibody 24284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols