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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of HepG2 using 66968-1-Ig (same clone as 66968-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of HeLa using 66968-1-Ig (same clone as 66968-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of HEK-293 using 66968-1-Ig (same clone as 66968-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    WB analysis of A431 using 66968-1-Ig (same clone as 66968-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    IF Staining of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PARD3 Monoclonal antibody (66968-1-Ig)

    FC experiment of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

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PARD3 Monoclonal antibody, PBS Only

PARD3 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66968-1-PBS
Clone No.1D2B2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66968-1-PBS targets PARD3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARD3 fusion protein Ag7656 相同性解析による交差性が予測される生物種
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Calculated molecular weight 151 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC011711
Gene Symbol PARD3
Gene ID (NCBI) 56288
RRIDAB_2882290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TEW0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.