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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PARG Monoclonal antibody

PARG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10G10

Cat no : 67946-1-Ig

Synonyms

PARG, PARG99

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARG Monoclonal antibody (67946-1-Ig)

    WB analysis using 67946-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67946-1-Ig (PARG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARG Monoclonal antibody (67946-1-Ig)

    IHC staining of human breast cancer using 67946-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARG Monoclonal antibody (67946-1-Ig)

    IHC staining of human breast cancer using 67946-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PARG Monoclonal antibody (67946-1-Ig)

    IF Staining of A431 using 67946-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PARG antibody (67946-1-Ig, Clone: 1H10G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67946-1-Ig targets PARG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PARG fusion protein Ag27288 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) glycohydrolase
Calculated molecular weight 111 kDa
Observed molecular weight111 kDa
GenBank accession numberBC052966
Gene symbol PARG
Gene ID (NCBI) 8505
RRIDAB_2918698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PARG antibody 67946-1-IgDownload protocol
IHC protocol for PARG antibody 67946-1-IgDownload protocol
IF protocol for PARG antibody 67946-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols