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  • Featured Product
  • KD/KO Validated

PARK2/Parkin Monoclonal antibody, PBS Only

PARK2/Parkin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A7

Cat no : 66674-1-PBS

Synonyms

Parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31, E3 ubiquitin-protein ligase parkin

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    WB analysis of SH-SY5Y using 66674-1-Ig (same clone as 66674-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    WB analysis of U-251 using 66674-1-Ig (same clone as 66674-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    WB analysis of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using PARK2/Parkin Monoclonal antibody (66674-1-Ig)

    IF Staining of RAW 264.7 using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody (66674-1-Ig, Clone: 2H5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66674-1-PBS targets PARK2/Parkin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PARK2/Parkin fusion protein Ag5179 相同性解析による交差性が予測される生物種
Full Name Parkinson disease (autosomal recessive, juvenile) 2, parkin
Calculated molecular weight 52 kDa
Observed molecular weight 42-52 kDa
GenBank accession numberBC022014
Gene symbol Parkin
Gene ID (NCBI) 5071
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.