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  • Featured Product
  • KD/KO Validated

PARK7/DJ-1 Recombinant antibody

PARK7/DJ-1 Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230124B7

Cat no : 82913-2-RR

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PARK7/DJ-1 Recombinant antibody (82913-2-RR)

    WB analysis of HEK-293 using 82913-2-RR

    WB result of PARK7/DJ-1 antibody (82913-2-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARK7/DJ-1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PARK7/DJ-1 Recombinant antibody (82913-2-RR)

    WB analysis using 82913-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82913-2-RR (PARK7/DJ-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PARK7/DJ-1 Recombinant antibody (82913-2-RR)

    IP experiment of HeLa using 82913-2-RR

    IP result of anti-PARK7/DJ-1 (IP:82913-2-RR, 4ug; Detection:82913-2-RR 1:2000) with HeLa cells lysate 1280 ug.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PARK7/DJ-1 Recombinant antibody (82913-2-RR)

    IF Staining of SH-SY5Y using 82913-2-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PARK7/DJ-1 antibody (82913-2-RR, Clone: 230124B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using PARK7/DJ-1 Recombinant antibody (82913-2-RR)

    FC experiment of HeLa using 82913-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-2-RR, Clone:230124B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82913-2-RR

    Affinity and Kinetic Characterization of 82913-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82913-2-RR against Human PARK7/DJ-1 were performed. The affinity constant is 7.89 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, BxPC-3 cells, Jurkat cells, HSC-T6 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82913-2-RR targets PARK7/DJ-1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARK7/DJ-1 fusion protein Ag2287 相同性解析による交差性が予測される生物種
Full Name Parkinson disease (autosomal recessive, early onset) 7
Calculated molecular weight 189 aa, 20 kDa
Observed molecular weight25 kDa
GenBank accession numberBC008188
Gene symbol PARK7
Gene ID (NCBI) 11315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

Protocols

Product Specific Protocols
WB protocol for PARK7/DJ-1 antibody 82913-2-RRDownload protocol
IF protocol for PARK7/DJ-1 antibody 82913-2-RRDownload protocol
IP protocol for PARK7/DJ-1 antibody 82913-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols