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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PARK7/DJ-1 Recombinant antibody, PBS Only

PARK7/DJ-1 Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

230124B3

Cat no : 82913-3-PBS

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARK7/DJ-1 Recombinant antibody (82913-3-RR)

    WB analysis using 82913-3-RR (same clone as 82913-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82913-3-RR (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82913-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PARK7/DJ-1 Recombinant antibody (82913-3-RR)

    FC experiment of HeLa using 82913-3-RR (same clone as 82913-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-3-RR, Clone:230124B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82913-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82913-3-RR

    Affinity and Kinetic Characterization of 82913-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82913-3-RR against Human PARK7/DJ-1 were performed. The affinity constant is 1.02 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 82913-3-PBS is PARK7/DJ-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARK7/DJ-1 fusion protein Ag2287 相同性解析による交差性が予測される生物種
Full Name Parkinson disease (autosomal recessive, early onset) 7
Calculated molecular weight 189 aa, 20 kDa
GenBank accession numberBC008188
Gene symbol PARK7
Gene ID (NCBI) 11315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.